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What Is a Temperature and Humidity Environmental Chamber with CO₂?

Nov 14, 2025

When industries need to understand how materials, electronics, food packaging, or biological samples behave in real-world environments, they rely on equipment that can reproduce temperature, humidity, and gas conditions with scientific accuracy. A Temperature and Humidity Environmental Chamber with CO₂ provides exactly this capability-combining climate simulation with adjustable carbon-dioxide concentrations to meet strict global standards such as IEC 60068-2-38, IEC 60068-2-78, ASTM D2247, ASTM D1735, ISO 187, ICH Q1A (R2), and ICH Q1B.

 

LIB Temperature and Humidity Environmental Chamber with CO₂ systems are engineered to deliver precise, repeatable, and certification-ready test results for electronics, polymers, coatings, pharmaceuticals, and agricultural research. With ranges such as –60 ℃ to +150 ℃, 10–98 % RH, and 0–20 % CO₂, the chamber supports both long-term stability evaluations and accelerated stress testing.

 

Introduction to Temperature and Humidity Environmental Chamber with CO₂


 

A Temperature and Humidity Environmental Chamber with CO₂ is designed to reproduce complex environments by controlling temperature, humidity, and carbon-dioxide levels in a sealed test space.These chambers allow researchers to study how materials and devices respond to combined climatic and gas-exposure stresses, which is essential in electronics, coatings, packaging, and biological sciences.

 

The chamber creates stable temperature conditions by integrating refrigeration, heating, and airflow modules that work together to maintain precise values such as ±0.5 ℃ fluctuation. Humidity is controlled by evaporative humidification and dehumidification paths, allowing accurate regulation from 10 % to 98 % RH with ±1 % to ±1.5 % RH accuracy. These factors ensure that test results are consistent and reproducible.

 

Adding CO₂ extends the testing capability far beyond conventional climate chambers. By simulating high-CO₂ industrial atmospheres, low-CO₂ storage conditions, or agricultural growth environments, the chamber becomes a versatile scientific tool. It supports testing from material corrosion under ISO 187 to pharmaceutical stability studies following ICH Q1A (R2).

 

In many cases, CO₂ concentration is as important as temperature or humidity. Food packaging materials may degrade faster in acidic gas environments; electronics may show corrosion or solder fatigue when exposed to elevated CO₂; biological samples may require specific CO₂ levels for cultivation. The chamber enables these controlled experiments with accuracy and consistency.

 

How Does a Temperature and Humidity Environmental Chamber with CO₂ Precisely Control Carbon Dioxide?


 

The chamber maintains accurate CO₂ levels using a closed-loop detection and control mechanism.At the center of this system is an infrared CO₂ sensor, which provides ±0.4 % accuracy and continuously measures real-time gas concentration inside the chamber.

To adjust CO₂ levels, the chamber uses a combination of precision solenoid valves and micro-flow controllers.

 

When CO₂ concentration drops below the setpoint, valves open to allow controlled gas injection from a cylinder or centralized gas supply. When concentration is high, the exhaust system dilutes the environment until measurements return to the programmed value.

 

Air circulation is essential for maintaining gas consistency. High-efficiency fans distribute air in multiple directions, ensuring that temperature, humidity, and CO₂ remain uniform throughout the test space-with gradients typically less than 1.5 ℃ across chamber volume.

 

Safety is integrated into the CO₂ control system. Over-concentration alarms, leakage-prevention sealing, and gas-tight construction protect operators and maintain testing integrity. These features make the system suitable for applications ranging from electronic reliability tests to seed germination and tissue-culture studies.

 

Temperature and Humidity Environmental Chamber with CO₂

Temperature and Humidity Environmental Chamber with CO₂

Model

CO-100

Internal Dimension (mm)

100*100*100 D*W*H

Overall Dimension (mm)

2100*1100*1700 D*W*H

Interior Volume (Liter)

100L

Temperature Range

-60℃ ~ +150 ℃(customized)

Cooling Rate

1 ℃ / min, average

Heating Rate

3 ℃ / min, average

Temperature Accuracy

± 0.5 ℃

Heat load

1000W

Humidity Range

10% ~ 98% RH(customized)

Humidity Accuracy

± 1% RH~± 1.5% RH

CO2 Range

0%~20%

CO2 Accuracy

<± 0.4% RH

Controller

Programmable color LCD touch screen controller,Ethernet connection, PC Link

Exterior Material

Steel Plate with protective coating

Interior Material

SUS304 stainless steel

 

Procedure For Testing Food Packaging Materials Using The LIB Temperature and Humidity Environmental Chamber with CO₂


 

Food packaging materials-such as films, laminates, trays, and coated papers-are affected by temperature changes, humidity exposure, and CO₂-rich environments during storage and transportation. Climate testing is used to evaluate moisture resistance, gas-barrier stability, deformation, and aging behavior before products enter mass production. The test helps assess barrier performance, shelf-life reliability, and material suitability under standardized environmental conditions.

 

Type Type 1 Type 2 Type 3
Name Temperature & Humidity Chamber Temperature, Humidity & CO₂ Chamber Multi-Gas Environmental Chamber
Model TH-100, TH-250, TH-500 THC-100, THC-250, THC-500 MGC-100, MGC-250, MGC-500
Test Standard ASTM D2247; ASTM D1735 IEC 60068-2-78; ICH Q1A(R2) ISO 187; ICH Q1B (Light + CO₂)
Function Temp (–20℃ ~ +150℃); Humid (10%~98%RH) Temp (–20℃ ~ +150℃); Humid (10%~98%RH); CO₂ (0–20%) Temp (–20℃ ~ +150℃); Humid (10%~98%RH); Multi-gas exposure

 

Temperature and Humidity Environmental Chamber with CO₂

Temperature and Humidity Environmental Chamber with CO₂
Test Area CO₂ Sensor

Step 1: Prepare The Packaging Samples

Cut packaging films or containers into required dimensions and record weight, thickness, and visual condition before testing.
LIB Advantage: Stainless-steel SUS304 shelves ensure stable placement and optimal airflow around all sample surfaces.

 

Step 2: Define Test Conditions

Select test conditions according to ASTM D2247, ASTM D1735, IEC 60068-2-78, or ICH Q1B. Set values such as 40℃ / 75%RH or 5% CO₂ to accelerate humidity and gas exposure.
LIB Advantage: High-precision sensors maintain temperature fluctuation within ±0.5℃ and humidity within ±1–1.5%RH.

 

Step 3: Set Chamber Parameters

Program temperature, humidity, and CO₂ levels through the 7-inch touchscreen controller. Choose ramp-soak cycles, long-term stability tests, or accelerated aging profiles.
LIB Advantage: CO₂ is precisely controlled through NDIR sensors and micro-flow valves for uniform gas distribution.

 

Step 4: Start The Exposure Test

Place samples evenly on shelves to avoid overlap and begin the test. The chamber runs continuously for hours or days depending on required aging duration.
LIB Advantage: Multi-directional air circulation ensures uniformity with temperature deviation ≤1.5℃ across the workspace.

 

Step 5: Inspect And Record Results

After exposure, remove samples and conduct appearance checks, weight-change testing, barrier-property analysis, or mechanical evaluation. Export all environmental data for reporting.
LIB Advantage: USB/Ethernet export provides CSV/PDF data for QA teams, audits, or regulatory documentation.

 

High-Uniformity Airflow Design

Ensures stable temperature, humidity, and CO₂ distribution.

 

Stainless-Steel Interior Chamber

Corrosion-resistant SUS304 construction supports long-term food-contact material testing.

 

Real-Time Data Logging System

Continuously tracks environmental parameters with automatic alarm storage.

 

Temperature and Humidity Environmental Chamber with CO₂ for CO₂-Conditioned Testing

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Temperature and Climate Test Chamber

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3. Temperature Explosion Proof Test Chamber

4. Durable Lab Vacuum Drying Oven

5. Energy-Efficient Battery Thermal Test Chamber

6. Programmable 2-Zone And 3-Zone Vertical Thermal Shock Test Chamber

7. High-Performance Cryogenic Chamber

8. Advanced Vibration Chamber

 

Convenient Services of LIB for Temperature and Humidity Environmental Chamber with CO₂


 

LIB provides comprehensive support services that cover customization, delivery, installation, and long-term operation. Any chamber can be tailored-including CO₂ range configuration, multi-gas inlet design, chamber volume (50 L–1000 L), cable-port sizing, shelves, and extended temperature–humidity envelopes-allowing users to meet strict testing requirements without redesigning their workflow.

LIB ensures fast and secure global delivery supported by professional installation teams.


Each chamber is packed with reinforced protection, shipped within 7–15 days, and installed onsite by trained technicians who perform calibration, power-up testing, and operator instruction. This reduces setup time and prevents operational errors during initial use.

LIB offers a reliable after-sales system designed for long-term equipment performance.


The company provides a 3-year warranty, lifetime technical support, and 24/7 English-speaking service for customers worldwide. Any malfunction that cannot be resolved during the warranty period is replaced free of charge, ensuring uninterrupted laboratory operation.

LIB also supports users with preventive-maintenance guidance and technical upgrades.


Services include humidity-system cleaning, CO₂-sensor verification, annual calibration recommendations, and controller-software updates, ensuring the chamber maintains accurate, compliant test performance throughout its lifespan.

 

Contact LIB Industry to develop a professional and efficient solution tailored to your specific standards and stringent requirements.

 

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